5-Amino-1MQ

Specification

Mechanism of action

5-Amino-1MQ belongs to the selective inhibitors of nicotinamide N-methyltransferase (NNMT). The enzyme methylates nicotinamide to 1-methylnicotinamide and sits at the junction of nicotinamide metabolism and NAD+ homeostasis. Published structure-activity studies characterise methylquinolinium derivatives as membrane-permeable NNMT inhibitors, and 5-Amino-1MQ is a reference compound in that series. In metabolic research models the substance is used to modulate how NNMT activity affects energy turnover, adipocyte differentiation and cellular NAD+ availability. Laboratories that buy 5-Amino-1MQ take the NNMT inhibitor at 10 mg per vial.

Solution and handling

As a small molecule, 5-Amino-1MQ is prepared in the laboratory in a solvent suited to the assay matrix. Compared with peptide lyophilisates, the substance is often soluble in organic or mixed solvent systems. The laboratory running the work sets the solvent and the final concentration in the protocol. After preparation, the solution is transferred promptly into enzyme or cell assays. Until use, the material is protected from direct light. Volume figures are not part of this description.

In vitro research

In in vitro research, laboratories use 5-Amino-1MQ as a reference inhibitor in NNMT enzyme assays and in cell-based metabolic models. Adipocyte, hepatocyte and myogenic cell lines are suited to testing how inhibition of NNMT activity changes nicotinamide flux, methylation products and downstream NAD+-dependent reactions. Membrane permeability of the methylquinolinium series makes the compound practical in intact cells, compared with purely cytosolic test systems. Concentration-response curves and selectivity comparisons with other NNMT ligands are typical experimental setups. Primary literature on selective NNMT inhibitors: Neelakantan et al., PubMed.

Quality and analytics

Each batch of 5-Amino-1MQ is tested for purity by HPLC, and the content is ≥99 %. An identity check confirms the expected methylquinolinium structure. Results are documented per batch and provide a reliable basis for reproducible enzyme and cell experiments. Also in the metabolic catalogue: MOTS-c for mitochondrial signalling peptides and SS-31 for mitochondrial membrane research.

Available amounts per vial: 5 mg, 10 mg, 50 mg. Box of 10 vials.