CJC-1295 (Mod GRF 1-29) 10 mg

Specification

Mechanism of action

CJC-1295 without DAC, also known as Mod GRF 1-29, is a tetrasubstituted analog of GRF(1-29)-NH2 with D-Ala2, Gln8, Ala15 and Leu27 and acts as a GHRH receptor agonist. It carries no DAC modification (Drug Affinity Complex) and no albumin-binding extension. In research, it is used in models of GHRH receptor pharmacology and of the GH axis; receptor binding and signal transduction are studied in endocrine cell models in vitro. As a defined reference material, CJC-1295 supports comparable experimental series in which ligand binding, second-messenger response, and downstream signaling are recorded. Positive findings in cell models describe binding to the GHRH receptor and activation of downstream signaling cascades. Researchers who buy CJC-1295 use this 10-vial kit as a defined reference for in-vitro work.

Reconstitution and handling

The lyophilized powder is reconstituted in the laboratory with a suitable sterile solvent. The resulting solution is processed promptly and protected from direct light during handling. Volume and concentration choices follow the experimental plan of the receiving facility; this data sheet makes no recommendations on them. A clean workspace and calibrated pipettes support the reproducibility of results. Fresh aliquots are prepared for each assay series so that conditions within a measurement run remain constant.

In-vitro research

In in-vitro research, CJC-1295 without DAC serves as a reference material for GHRH receptor and GH axis studies. Endocrine cell models are used to examine binding, receptor activation, and downstream signal transduction. Studies on the DAC variant, such as Teichman et al. (2006), describe a different molecule and cannot be transferred to the No-DAC form. Defined purity and lot-specific testing make the molecule a reproducible anchor for positive controls and comparison runs in controlled experimental setups. The four substitutions distinguish Mod GRF 1-29 from native GRF(1-29) and are listed in the sequence above.

Quality and analytics

Each lot is tested for purity by HPLC and reaches at least ≥99 %. A complementary identity check confirms the Mod GRF 1-29 sequence without DAC. Testing is performed per lot and applies uniformly to all vials in a box; results are recorded for the respective lot. The reported molecular weight of 3.368 kDa refers to the No-DAC form. The documented values refer to the tested lot and the associated vials in the box.